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Cytokine studies in the generation and treatment of IgE-mediated allergy
Stockholm University, Faculty of Science, The Wenner-Gren Institute, Immunology.
2002 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

So called Th2 cytokines, such as IL-4 and IL-13 are responsible for the formation of IgE antibodies, the effector antibody in atopic allergy. Cytokines of Th1 type, for example IFN-g, inhibit these responses. This thesis describes work aimed at elucidating the cytokine production underlying IgE-mediated allergy.

Allergen specific immunotherapy (SIT) is an effective form of treatment for IgE-mediated allergy. The cytokine production in peripheral blood mononuclear cells (PBMC) taken from atopic individuals undergoing allergen SIT or placebo treatment was investigated. An increased number of IL-4- and IL-13-producing PBMC was observed during the pollen season in placebo-treated individuals, whereas in patients receiving allergen SIT, this increase was absent. Thus, in line with other studies, our findings suggest that allergen SIT exerts its effect on allergen specific T cells.

Most IgE-mediated allergies develop during early childhood. Therefore, it is of major importance to understand the regulation of these initial immune responses. Cord blood mononuclear cells (CBMC) were collected from newborns and divided into three different groups, those with double atopic, maternal atopic or no atopic heredity. Following PHA stimulation in vitro, the ratio of IL-4-/IFN-g-producing CBMC was significantly higher in the group with double atopic heredity than in the group without. This suggests a strong genetic influence on cytokine-producing cells in the newborn. Large numbers of IL-12-producing CBMC were induced following allergen stimulation. This finding is in contrast to the general idea about deficient Th1- and Th1-inducing responses in cord blood.

The soluble form of CD14, an endotoxin receptor involved in innate immune responses, have been negatively associated with levels of total serum IgE. We investigated whether any differences in soluble or membrane bound CD14 could be detected already in cord blood, and whether these levels were related to those in the mothers or in the same child at two years of age. We could not confirm any negative association between total serum IgE levels and sCD14. With regard to atopy, higher levels of sCD14 were found in atopic mothers compared to non-atopic, and the same was true for the cord blood of their corresponding children. The membrane bound form of CD14 was higher on cord blood cells than on cells taken from mothers or two-year-olds, whereas the opposite was observed for sCD14. These findings suggest that the initial role of CD14 might be in the early maturation and differentiation events of the immune system.

Place, publisher, year, edition, pages
Stockholm: Stockholm University , 2002. , 77 p.
Keyword [en]
Cytokines, Hypersensitivity, Allergy
Keyword [sv]
Allergi
National Category
Immunology
Research subject
Immunology
Identifiers
URN: urn:nbn:se:su:diva-142856Libris ID: 8396654ISBN: 91-7265-413-9 (print)OAI: oai:DiVA.org:su-142856DiVA: diva2:1095141
Public defence
2002-03-08, 10:00 (English)
Opponent
Note

Härtill 5 uppsatser

Available from: 2017-05-12 Created: 2017-05-12 Last updated: 2017-10-20Bibliographically approved

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