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Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers
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Number of Authors: 56
2017 (English)In: Nature Methods, ISSN 1548-7091, E-ISSN 1548-7105, Vol. 14, no 4, 443-449 p.Article in journal (Refereed) Published
Abstract [en]

X-ray crystallography at X-ray free-electron laser sources is a powerful method for studying macromolecules at biologically relevant temperatures. Moreover, when combined with complementary techniques like X-ray emission spectroscopy, both global structures and chemical properties of metalloenzymes can be obtained concurrently, providing insights into the interplay between the protein structure and dynamics and the chemistry at an active site. The implementation of such a multimodal approach can be compromised by conflicting requirements to optimize each individual method. In particular, the method used for sample delivery greatly affects the data quality. We present here a robust way of delivering controlled sample amounts on demand using acoustic droplet ejection coupled with a conveyor belt drive that is optimized for crystallography and spectroscopy measurements of photochemical and chemical reactions over a wide range of time scales. Studies with photosystem IIII, the phytochrome photoreceptor, and ribonucleotide reductase R2 illustrate the power and versatility of this method.

Place, publisher, year, edition, pages
2017. Vol. 14, no 4, 443-449 p.
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Biological Sciences
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URN: urn:nbn:se:su:diva-142628DOI: 10.1038/nmeth.4195ISI: 000397900500029PubMedID: 28250468OAI: oai:DiVA.org:su-142628DiVA: diva2:1096312
Available from: 2017-05-17 Created: 2017-05-17 Last updated: 2017-05-17Bibliographically approved

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Lebrette, HugoSrinivas, Vivek
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