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Expression of a Drosophila glutathione transferase in Arabidopsis confers the ability to detoxify the environmental pollutant, and explosive, 2,4,6-trinitrotoluene
Stockholm University, Faculty of Science, Department of Neurochemistry.
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Number of Authors: 7
2017 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 214, no 1, 294-303 p.Article in journal (Refereed) Published
Abstract [en]

The explosive 2,4,6-trinitrotoluene (TNT) is a significant, global environmental pollutant that is both toxic and recalcitrant to degradation. Given the sheer scale and inaccessible nature of contaminated areas, phytoremediation may be a viable clean-up approach. Here, we have characterized a Drosophila melanogaster glutathione transferase (DmGSTE6) which has activity towards TNT. Recombinantly expressed, purified DmGSTE6 produces predominantly 2-glutathionyl-4, 6-dinitrotoluene, and has a 2.5-fold higher Maximal Velocity (Vmax), and five-fold lower Michaelis Constant (Km) than previously characterized TNT-active Arabidopsis thaliana (Arabidopsis) GSTs. Expression of DmGSTE6 in Arabidopsis conferred enhanced resistance to TNT, and increased the ability to remove TNT from contaminated soil relative to wild-type plants. Arabidopsis lines overexpressing TNT-active GSTs AtGST-U24 and AtGST-U25 were compromised in biomass production when grown in the absence of TNT. This yield drag was not observed in the DmGSTE6-expressing Arabidopsis lines. We hypothesize that increased levels of endogenous TNT-active GSTs catalyse excessive glutathionylation of endogenous substrates, depleting glutathione pools, an activity that DmGST may lack. In conclusion, DmGSTE6 has activity towards TNT, producing a compound with potential for further biodegradation. Selecting or manipulating plants to confer DmGSTE6-like activity could contribute towards development of phytoremediation strategies to clean up TNT from polluted military sites.

Place, publisher, year, edition, pages
2017. Vol. 214, no 1, 294-303 p.
Keyword [en]
2, 4, 6-trinitrotoluene, Arabidopsis, conjugation, detoxification, Drosophila melanogaster, environmental pollutant, glutathione transferase, phytoremediation
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-142613DOI: 10.1111/nph.14326ISI: 000398130300028OAI: oai:DiVA.org:su-142613DiVA: diva2:1097281
Available from: 2017-05-22 Created: 2017-05-22 Last updated: 2017-05-22Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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