Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Hormonal changes over the spawning cycle in the female three-spined stickleback, Gasterosteus aculeatus
Stockholm University, Faculty of Science, Department of Zoology.ORCID iD: 0000-0003-0022-0698
Show others and affiliations
(English)Manuscript (preprint) (Other academic)
Abstract [en]

Female three-spined sticklebacks are batch spawners laying eggs in a nest built by the male. We sampled female sticklebacks at different time points, when they were ready to spawn and 6, 24, 48 and 72 hours post-spawning (hps) with a male. Following spawning, almost all females again had ovulated eggs at Day 3 post-spawning. At sampling, plasma, brain and pituitaries were collected, and the ovary was weighed. Circulating sex steroid hormones [testosterone (T), estradiol (E2), 17,20β-dihydroxypregn-4-en-3-one (17,20β-P) and 17,20β,21-trihydroxypregn-4-en-3-one (17,20β,21-P)] were measured by radioimmunoassay. Moreover, the mRNA levels of follicle-stimulating hormone (fsh-β) and luteinizing hormone (lh-β) in the pituitary, and of the gonadotropin-releasing hormones (GnRHs: gnrh2, gnrh3) and kisspeptin (kiss2) and its G protein-coupled receptor (gpr54) in the brain were measured by real-time qPCR.

Ovarian weights peaked in “ready to spawn” females, dropped after spawning, before again progressively increasing from 6 to 72 hps. Plasma T levels showed peaks at 24 and 48hps and decreased at 72hps, while E2 levels increased already at 6hps and remained at high levels up to 48hps. There was a strong positive correlation between T and E2 levels over the spawning cycle. Both progestogens were non-detectable in most samples. Pituitary lh-β mRNA levels showed a peak at 48hps, while fsh-β did not change. The neuropeptides and gpr54 did not show any changes.

Keyword [en]
sex steroids, gonadotropins, GnRH, spawning cycle, stickleback, teleost
National Category
Biological Sciences
Research subject
Functional Zoomorphology
Identifiers
URN: urn:nbn:se:su:diva-143271OAI: oai:DiVA.org:su-143271DiVA: diva2:1097526
Available from: 2017-05-22 Created: 2017-05-22 Last updated: 2017-06-01Bibliographically approved
In thesis
1.
The record could not be found. The reason may be that the record is no longer available or you may have typed in a wrong id in the address field.

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Roufidou, ChrysoulaBorg, Bertil
By organisation
Department of Zoology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

Total: 42 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf