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Ovarian fluid in the three-spined stickleback, Gasterosteus aculeatus: effects of egg overripening and sex steroid treatment
Stockholm University, Faculty of Science, Department of Zoology.ORCID iD: 0000-0003-0022-0698
Stockholm University, Faculty of Science, Department of Zoology.
(English)Manuscript (preprint) (Other academic)
Abstract [en]

In most fishes, including the three-spined stickleback, Gasterosteus aculeatus, the ovulated eggs are surrounded by the ovarian fluid (OF). OF is probably secreted by the ovarian lumen epithelium under stimulation by sex steroids. OF is believed to have several biological functions, including the maintance of the egg viability after ovulation. Female sticklebacks produce eggs in batches, and if spawning or spontaneous dropping of the eggs does not happen, they can become overripe. In the present study, OF properties were studied in overripe and non-overripe ovulated female sticklebacks, and the effects of testosterone (T), estradiol (E2), 17,20β-dihydroxypregn-4-en-3-one (17-20β-P) and progesterone (P4) on OF properties in females that were implanted with Silastic capsules into the abdominal cavity, after having spawned naturally were investigated. Overripe females had a diminished amount of OF, that had a higher percentage dry weight, higher protein concentration levels and lower viscocity compared to OF from non-overripe ovulated females. T delayed the next ovulation. Both T and 17,20β-P induced a significant increase of OF amount. However, the OF protein concentration was significantly increased only in 17,20β-P females. 1-D SDS-PAGE analysis showed that OF contains several proteins, with high individual variability but with no consistent differences between groups. Some of the OF proteins appeared to correspond to proteins from the eggs. The results suggest that secretion of OF may be under the control of 17,20β-P and that changes in its properties are related to the overripening of eggs.

Keyword [en]
ovarian fluid, overripening, sex steroids, stickleback, teleosts
National Category
Biological Sciences
Research subject
Functional Zoomorphology
Identifiers
URN: urn:nbn:se:su:diva-143276OAI: oai:DiVA.org:su-143276DiVA: diva2:1097592
Available from: 2017-05-22 Created: 2017-05-22 Last updated: 2017-06-01Bibliographically approved
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