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Sensitive magnetic biodetection using magnetic multi-core nanoparticles and RCA coils
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Number of Authors: 15
2017 (English)In: Journal of Magnetism and Magnetic Materials, ISSN 0304-8853, E-ISSN 1873-4766, Vol. 427, 14-18 p.Article in journal (Refereed) Published
Abstract [en]

We use functionalized iron oxide magnetic multi-core particles of 100 nm in size (hydrodynamic particle diameter) and AC susceptometry (ACS) methods to measure the binding reactions between the magnetic nanoparticles (MNPs) and bio-analyte products produced from DNA segments using the rolling circle amplification (RCA) method. We use sensitive induction detection techniques in order to measure the ACS response. The DNA is amplified via RCA to generate RCA coils with a specific size that is dependent on the amplification time. After about 75 min of amplification we obtain an average RCA coil diameter of about 1 mu m. We determine a theoretical limit of detection (LOD) in the range of 11 attomole (corresponding to an analyte concentration of 55 fM for a sample volume of 200 mu L) from the ACS dynamic response after the MNPs have bound to the RCA coils and the measured ACS readout noise. We also discuss further possible improvements of the LOD.

Place, publisher, year, edition, pages
2017. Vol. 427, 14-18 p.
Keyword [en]
Magnetic nanoparticles, Magnetic multi-core particles, AC susceptometry, RCA coil, Differential induction coil, Biodetection
National Category
Biological Sciences Physical Sciences
Identifiers
URN: urn:nbn:se:su:diva-142605DOI: 10.1016/j.jmmm.2016.10.041ISI: 000397199400004OAI: oai:DiVA.org:su-142605DiVA: diva2:1097973
Available from: 2017-05-23 Created: 2017-05-23 Last updated: 2017-05-23Bibliographically approved

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Nilsson, Mats
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Department of Biochemistry and BiophysicsScience for Life Laboratory (SciLifeLab)
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