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A Magnetic Nanobead-Based Read-Out Procedure for Rapid Detection of DNA Molecules
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab). Uppsala University, Sweden.
Number of Authors: 3
2017 (English)In: Journal of Nanoscience and Nanotechnology, ISSN 1533-4880, E-ISSN 1533-4899, Vol. 17, no 4, 2861-2864 p.Article in journal (Refereed) Published
Abstract [en]

The presented measurement and data analysis procedure reduces the read-out time for the volumeamplified magnetic nanobead detection assay from similar to 30 min to only 2 min, providing fast, sensitive detection of DNA molecules. The molecular detection and amplification protocol was verified using samples containing rolling circle-amplified DNA products formed from synthetic Vibrio cholerae target DNA, with a limit of detection of 5 pM. The developed read-out method could be used to rapidly identify pathogens in a variety of applications including target screening in hospitals with limited resources, in out-patient settings and in the field.

Place, publisher, year, edition, pages
2017. Vol. 17, no 4, 2861-2864 p.
Keyword [en]
Brownian Relaxation, DNA Macromolecules, Magnetic Nanobeads, Rapid Detection, Rolling Circle Amplification
National Category
Biological Sciences Nano Technology
Identifiers
URN: urn:nbn:se:su:diva-142606DOI: 10.1166/jnn.2017.13877ISI: 000397853900111OAI: oai:DiVA.org:su-142606DiVA: diva2:1097977
Available from: 2017-05-23 Created: 2017-05-23 Last updated: 2017-05-23Bibliographically approved

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