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Characterization of fast-tumbling isotropic bicelles by PFG diffusion NMR
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Number of Authors: 3
2017 (English)In: Magnetic Resonance in Chemistry, ISSN 0749-1581, E-ISSN 1097-458X, Vol. 55, no 5, 395-404 p.Article, review/survey (Refereed) Published
Abstract [en]

Small isotropic bicelles are versatile membrane mimetics, which, in contrast tomicelles, provide a lipid bilayer and are at the same time suitable for solution-state NMR studies. The lipid composition of the bilayer is flexible allowing for incorporation of various head groups and acyl chain types. In bicelles, lipids are solubilized by detergents, which are localized in the rimof the disk-shaped lipid bilayer. Bicelles have been characterized by a broad array of biophysical methods, pulsed-field gradient NMR (PFG NMR) being one of them. PFG NMR can readily be used to measure diffusion coefficients of macromolecules. It is thus employed to characterize bicelle size and morphology. Even more importantly, PFG NMR can be used to study the degree of protein association to membranes. Here, we present the advances that have been made in producing small, fast-tumbling isotropic bicelles from a variety of lipids and detergents, together with insights on the morphology of such mixtures gained from PFG NMR. Furthermore, we review approaches to study protein-membrane interaction by PFG NMR.

Place, publisher, year, edition, pages
2017. Vol. 55, no 5, 395-404 p.
Keyword [en]
morphology, diffusion, bilayer association, membrane mimetics, detergent, lipid
National Category
Biological Sciences
Research subject
Biophysics
Identifiers
URN: urn:nbn:se:su:diva-143384DOI: 10.1002/mrc.4399ISI: 000400599500003PubMedID: 26662467OAI: oai:DiVA.org:su-143384DiVA: diva2:1105212
Available from: 2017-06-02 Created: 2017-06-02 Last updated: 2017-09-08Bibliographically approved

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