Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Calcium improves apoplastic-cytosolic ion homeostasis in salt-stressed Vicia faba leaves
Stockholm University, Faculty of Science, Department of Ecology, Environment and Plant Sciences.
Stockholm University, Faculty of Science, Department of Ecology, Environment and Plant Sciences.
Show others and affiliations
Number of Authors: 6
2017 (English)In: Functional Plant Biology, ISSN 1445-4408, E-ISSN 1445-4416, Vol. 44, no 5, 515-524 p.Article in journal (Refereed) Published
Abstract [en]

Salinity disturbs both apoplastic and cytosolic Ca2+ and pH ([Ca2+](apo), [Ca2+](cyt), pH(apo) and pH(cyt)) homeostasis, and decreases plant growth. Seedlings of Vicia faba L. cv. Fuego were cultivated in hydroponics for 7 days under control, salinity (S), extra Ca (Ca) or salinity with extra Ca (S+Ca) conditions. The [Ca2+](apo), and pH(apo) in the leaves were then recorded in parallel by a pseudoratiometric method, described here for the first time. Lower [Ca2+](apo) and higher pH(apo) were obtained under salinity, whereas extra Ca supply increased the [Ca2+](apo) and acidified the pH(apo). Moreover, the ratiometric imaging recorded that [Ca2+](cyt) and pH(cyt) were highest in S+Ca plants and lowest in control plants. After all pretreatments, direct addition of NaC6H11O7 to leaves induced a decrease in [Ca2+](apo) in control and S+Ca plants, but not in S and Ca plants, and only slightly affected pH(apo). Addition of NaCl increased [Ca2+](cyt) in protoplasts from all plants but only transiently in protoplasts from S+Ca plants. Addition of NaCl decreased pH(cyt) in protoplasts from Ca-pretreated plants. We conclude that Ca supply improves both apoplastic and cytosolic ion homeostasis. In addition, NaC6H11O7 probably causes transport of Ca from the apoplast into the cytosol, thereby leading to a higher resting [Ca2+](cyt).

Place, publisher, year, edition, pages
2017. Vol. 44, no 5, 515-524 p.
Keyword [en]
Calcium Green, fluorescence microscopy, Lucifer Yellow, ratio imaging, salinity stress
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-143854DOI: 10.1071/FP15381ISI: 000398845700005OAI: oai:DiVA.org:su-143854DiVA: diva2:1105733
Available from: 2017-06-05 Created: 2017-06-05 Last updated: 2017-06-05Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Lindberg, Sylvia
By organisation
Department of Ecology, Environment and Plant Sciences
In the same journal
Functional Plant Biology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 2 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf