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Mechanistic design of structural elements controlling proton pumping in cytochrone c oxidase
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
2001 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Cytochrome c oxidase is the last component of the respiratory chain in mitochondria and many bacteria, where it catalyses the reduction of O2 to water. The structures of cytochrome c oxidases from a number of organisms such as bacteria and cow, are known and show remarkable similarities in both structure and function.

The enzyme is bound to the inner mitochondrial membrane or in the cell membrane of bacteria. During reduction of dioxygen, protons are taken up from the inside of the membrane. In addition, the excess energy released in the oxygen-reduction reaction is used to pump protons across the membrane. The energy conserved by generation of the proton gradient across the membrane is utilised for synthesis of ATP by ATP synthase. The reduction of O2 in the enzyme must be carefully controlled in order to utilise the free energy of the reaction for proton pumping and to avoid the release of partially-reduced O2 intermediates which, if released, may be harmful to the cell.

In this work, the mechanisms controlling the electron- and proton-transfer events during reduction of dioxygen in cytochrome c oxidase have been studied. The main focus has been on cytochrome c oxidase from bovine heart and from the bacterium Rhodobacter sphaeroides. Comparative studies have also been made with a similar enzyme from the thermophilic bacterium Rhodothermus marinus. The results from this work have been summarised in a model that describes the oxygen-reduction chemistry and its coupling to the proton-pumping machinery. 

Place, publisher, year, edition, pages
Stockholm: Department of Biochemistry and Biophysics, Stockholm University , 2001. , 65 p.
National Category
Biological Sciences
Research subject
Biogeochemistry
Identifiers
URN: urn:nbn:se:su:diva-144229ISBN: 91-7265-336-1 (print)OAI: oai:DiVA.org:su-144229DiVA: diva2:1112263
Public defence
2001-09-14, 10:00 (English)
Opponent
Note

Härtill 5 uppsatser

Stockholms universitetsbiblioteks retrospektiva digitalisering. Avhandlingar 1906-2003.

Available from: 2017-06-20 Created: 2017-06-20 Last updated: 2017-07-12Bibliographically approved

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Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
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  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
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  • Other locale
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  • asciidoc
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