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Chemical microenvironments and single-cell carbon and nitrogen uptake in field-collected colonies of Trichodesmium under different pCO(2)
Stockholm University, Faculty of Science, Department of Ecology, Environment and Plant Sciences.
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Number of Authors: 9
2017 (English)In: The ISME Journal, ISSN 1751-7362, E-ISSN 1751-7370, Vol. 11, no 6, 1305-1317 p.Article in journal (Refereed) Published
Abstract [en]

Gradients of oxygen (O-2) and pH, as well as small-scale fluxes of carbon (C), nitrogen (N) and O-2 were investigated under different partial pressures of carbon dioxide (pCO(2)) in field-collected colonies of the marine dinitrogen (N-2)-fixing cyanobacterium Trichodesmium. Microsensor measurements indicated that cells within colonies experienced large fluctuations in O-2, pH and CO2 concentrations over a day-night cycle. O-2 concentrations varied with light intensity and time of day, yet colonies exposed to light were supersaturated with O-2 (up to similar to 200%) throughout the light period and anoxia was not detected. Alternating between light and dark conditions caused a variation in pH levels by on average 0.5 units (equivalent to 15 nmol l(-1) proton concentration). Single-cell analyses of C and N assimilation using secondary ion mass spectrometry (SIMS; large geometry SIMS and nanoscale SIMS) revealed high variability in metabolic activity of single cells and trichomes of Trichodesmium, and indicated transfer of C and N to colony-associated non-photosynthetic bacteria. Neither O-2 fluxes nor C fixation by Trichodesmium were significantly influenced by short-term incubations under different pCO(2) levels, whereas N-2 fixation increased with increasing pCO(2). The large range of metabolic rates observed at the single-cell level may reflect a response by colony-forming microbial populations to highly variable microenvironments.

Place, publisher, year, edition, pages
2017. Vol. 11, no 6, 1305-1317 p.
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Biological Sciences
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URN: urn:nbn:se:su:diva-144797DOI: 10.1038/ismej.2017.15ISI: 000401323100002PubMedID: 28398346OAI: oai:DiVA.org:su-144797DiVA: diva2:1121831
Available from: 2017-07-12 Created: 2017-07-12 Last updated: 2017-07-12Bibliographically approved

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