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The Formation of Nanoparticles between Small Interfering RNA and Amphipathic Cell-Penetrating Peptides
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Number of Authors: 5
2017 (English)In: Molecular Therapy - Nucleic Acids, ISSN 2162-2531, E-ISSN 2162-2531, Vol. 7, 1-10 p.Article in journal (Refereed) Published
Abstract [en]

Cell-penetrating peptides (CPPs) are delivery vectors widely used to aid the transport of biologically active cargoes to intracellular targets. These cargoes include small interfering RNAs (siRNA) that are not naturally internalized by cells. Elucidating the complexities behind the formation of CPP and cargo complexes is crucial for understanding the processes related to their delivery. In this study, we used modified analogs of the CPP transportan10 and investigated the binding properties of these CPPs to siRNA, the formation parameters of the CPP/siRNA complexes, and their stabiliy to enzymatic degradation. We conclude that the pH dependent change of the net charge of the CPP may very well be the key factor leading to the high delivery efficiency and the optimal binding strength between CPPs to siRNAs, while the hydrophobicity, secondary structure of the CPP, and the positions of the positive charges are responsible for the stability of the CPP/siRNA particles. Also, CPPs with distinct hydrophobic and hydrophilic regions may assemble into nanoparticles that could be described as coreshell formulations.

Place, publisher, year, edition, pages
2017. Vol. 7, 1-10 p.
Keyword [en]
cell-penetrating peptides, siRNA, nanoparticles, degradation, complexation
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Neurosciences Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-144777DOI: 10.1016/j.omtn.2017.02.003ISI: 000401233700001OAI: oai:DiVA.org:su-144777DiVA: diva2:1127546
Available from: 2017-07-17 Created: 2017-07-17 Last updated: 2017-11-29Bibliographically approved

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Langel, Ülo
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Department of Neurochemistry
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Molecular Therapy - Nucleic Acids
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)NeurosciencesBiological Sciences

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