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Generation of a multipurpose Prdm16 mouse allele by targeted gene trapping
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
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Number of Authors: 12
2017 (English)In: Disease Models and Mechanisms, ISSN 1754-8403, E-ISSN 1754-8411, Vol. 10, no 7, 909-922 p.Article in journal (Refereed) Published
Abstract [en]

Gene trap mutagenesis is a powerful tool to create loss-of-function mutations in mice and other model organisms. Modifications of traditional gene trap cassettes, including addition of conditional features in the form of Flip-excision (FlEx) arrays to enable directional gene trap cassette inversions by Cre and Flpe site-specific recombinases, greatly enhanced their experimental potential. By taking advantage of these conditional gene trap cassettes, we developed a generic strategy for generating conditional mutations and validated this strategy in mice carrying a multipurpose allele of the Prdm16 transcription factor gene. We demonstrate that the gene trap insertion creates a null mutation replicating the Pierre Robin sequence-type cleft palate phenotype of other Prdm16 mutant mice. Consecutive breeding to Flpe and Emx1(IREScre) deleter mice spatially restricted Prdm16 loss to regions of the forebrain expressing the homeobox gene Emx1, demonstrating the utility of the technology for the analysis of tissue-specific gene functions.

Place, publisher, year, edition, pages
2017. Vol. 10, no 7, 909-922 p.
Keyword [en]
Conditional gene trap, Cleft palate, Mandible, Micrognathia, Pierre Robin sequence
National Category
Cell Biology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:su:diva-145334DOI: 10.1242/dmm.029561ISI: 000404963600008OAI: oai:DiVA.org:su-145334DiVA: diva2:1128798
Available from: 2017-07-28 Created: 2017-07-28 Last updated: 2017-07-28Bibliographically approved

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Dai, Qi
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Department of Molecular Biosciences, The Wenner-Gren Institute
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Cell BiologyMedical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

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CiteExportLink to record
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