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Graphene oxide nanosheets in complex with cell penetrating peptides for oligonucleotides delivery
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Materials and Environmental Chemistry (MMK).
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Materials and Environmental Chemistry (MMK).
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2017 (English)In: Biochimica et Biophysica Acta - General Subjects, ISSN 0304-4165, E-ISSN 1872-8006, Vol. 1861, no 9, 2334-2341 p.Article in journal (Refereed) Published
Abstract [en]

A new strategy for gene transfection using the nanocarrier of cell penetrating peptides (CPPs; PepFect14 (PF14) or PepFect14 (PF14) (PF221)) in complex with graphene oxide (GO) is reported. GO complexed with CPPs and plasmid (pGL3), splice correction oligonucleotides (SCO) or small interfering RNA (siRNA) are performed. Data show adsorption of CPPs and oligonucleotides on the top of the graphenic lamellar without any observed change of the particle size of GO. GO mitigates the cytotoxicity of CPPs and improves the material biocompatibility. Complexes of GO-pGL3-CPPs (CPPs; PF14 or PF221) offer 2.1–2.5 fold increase of the cell transfection compared to pGL3-CPPs (CPPs; PF14 or PF221). GO-SCO-PF14 assemblies effectively transfect the cells with an increase of > 10–25 fold compared to the transfection using PF14. The concentration of GO plays a significant role in the material nanotoxicity and the transfection efficiency. The results open a new horizon in the gene treatment using CPPs and offer a simple strategy for further investigations.

Place, publisher, year, edition, pages
2017. Vol. 1861, no 9, 2334-2341 p.
Keyword [en]
Graphene oxide, Cell penetrating peptides, Transfection, Gene delivery
National Category
Biological Sciences Chemical Sciences
Research subject
Inorganic Chemistry
Identifiers
URN: urn:nbn:se:su:diva-146293DOI: 10.1016/j.bbagen.2017.07.002ISI: 000408071800018OAI: oai:DiVA.org:su-146293DiVA: diva2:1136735
Available from: 2017-08-29 Created: 2017-08-29 Last updated: 2017-09-18Bibliographically approved

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Dowaidar, MoatazAbdelhamid, Hani NasserHällbrink, MattiasZou, XiaodongLangel, Ülo
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Department of NeurochemistryDepartment of Materials and Environmental Chemistry (MMK)
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