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Genome-wide identification of Grainy head targets in Drosophila reveals regulatory interactions with the POU domain transcription factor Vvl
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute. Sloan-Kettering Institute, USA.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute. Sloan-Kettering Institute, USA.
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Number of Authors: 9
2017 (English)In: Development, ISSN 0950-1991, E-ISSN 1477-9129, Vol. 144, no 17, 3145-3155 p.Article in journal (Refereed) Published
Abstract [en]

Grainy head (Grh) is a conserved transcription factor (TF) controlling epithelial differentiation and regeneration. To elucidate Grh functions we identified embryonic Grh targets by ChIP-seq and gene expression analysis. We show that Grh controls hundreds of target genes. Repression or activation correlates with the distance of Grh-binding sites to the transcription start sites of its targets. Analysis of 54 Grh-responsive enhancers during development and upon wounding suggests cooperation with distinct TFs in different contexts. In the airways, Grh-repressed genes encode key TFs involved in branching and cell differentiation. Reduction of the POU domain TF Ventral veins lacking (Vvl) largely ameliorates the airway morphogenesis defects of grh mutants. Vvl and Grh proteins additionally interact with each other and regulate a set of common enhancers during epithelial morphogenesis. We conclude that Grh and Vvl participate in a regulatory network controlling epithelial maturation.

Place, publisher, year, edition, pages
2017. Vol. 144, no 17, 3145-3155 p.
Keyword [en]
Drosophila, Airway, Chip-seq, Epithelial maturation, Grainy head, Microarray
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-147018DOI: 10.1242/dev.143297ISI: 000408568500014PubMedID: 28760809OAI: oai:DiVA.org:su-147018DiVA: diva2:1145391
Available from: 2017-09-28 Created: 2017-09-28 Last updated: 2017-09-28Bibliographically approved

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Yao, LiqunDai, QiMatsuda, RyoHosono, ChieSamakovlis, Christos
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