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Immunocytochemical demonstration of the neurosecretory systems containing putative moult-inhibiting hormone and hyperglycemic hormone in the eyestalk of brachyuran crustaceans
Stockholm University, Faculty of Science, Department of Zoology, Functional Morphology. Rheinische Friedrich-Wilhelms Universit├Ąt, Germany.
School of Biological Sciences University of Wales, Bangor, UK.
1988 (English)In: Cell and Tissue Research, ISSN 0302-766X, E-ISSN 1432-0878, Vol. 251, 3-12 p.Article in journal (Refereed) Published
Abstract [en]

By use of antisera raised against purified moultinhibiting (MIH) and crustacean hyperglycemic hormone (CHH) from Carcinus maenas, complete and distinct neurosecretory pathways for both hormones were demonstrated with the PAP and immunofluorescence technique. By double staining, employing a combination of silver-enhanced immunogold labelling and PAP, both antigens could be visualized in the same section. Immunoreactive structures were studied in Carcinus maenas, Liocarcinus puber, Cancer pagurus, Uca pugilator and Maja squinado. They were only observed in the X-organ sinus gland (SG) system of the eyestalks and consisted of MIH-positive perikarya, which were dispersed among the more numerous CHH-positive perikarya of the medulla terminalis X-organ (XO). The MIH-positive neurons form branching collateral plexuses adjacent to the XO and axons that are arranged around the CHH-positive central axon bundle of the principal XO-SG tract. In the SG, MIH-positive axon profiles and terminals, clustered around hemolymph lacunae, are distributed between the more abundant CHH-positive axon profiles and terminals. Colocalisation of MIH and CHH was never observed. The gross morphology of both neurosecretory systems was similar in all species examined, however, in U. pugilator and M. squinado immunostaining for MIH was relatively faint unless higher concentrations of antiserum were used. Possible reasons for this phenomenon as well as observed moult cycle-related differences in immunostaining are discussed.

Place, publisher, year, edition, pages
1988. Vol. 251, 3-12 p.
Keyword [en]
moult-inhibiting hormone, crustacean hyperglycemic hormone, crab, Carcinus maenas
National Category
Zoology
Research subject
Zoology
Identifiers
URN: urn:nbn:se:su:diva-147520DOI: 10.1007/BF00215441OAI: oai:DiVA.org:su-147520DiVA: diva2:1146317
Available from: 2017-10-02 Created: 2017-10-02 Last updated: 2017-10-04

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