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An inner nuclear membrane protein induces rapid differentiation of human induced pluripotent stem cells
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Neurochemistry.
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Number of Authors: 52017 (English)In: Stem Cell Research, ISSN 1873-5061, E-ISSN 1876-7753, Vol. 23, p. 33-38Article in journal (Refereed) Published
Abstract [en]

The ability of iPSCs (induced pluripotent stem cells) to generate any cell type in the body makes them valuable tools for cell replacement therapies. However, differentiation of iPSCs can be demanding, slowand variable. During differentiation chromatin is re-organized and silent dense heterochromatin becomes tethered to the nuclear periphery by processes involving the nuclear lamina and proteins of the INM(inner nuclearmembrane). The INM protein, Samp1 (Spindle AssociatedMembrane Protein 1) interacts with Lamin A/C and the INMprotein Emerin, which has a chromatin binding LEM(Lap2-Emerin-Man1)-domain. In this paperweinvestigate if Samp1 can play a role in the differentiation of iPSCs. Samp1 levels increased as differentiating iPSCs started to express Lamin A/C. Interestingly, even under pluripotent culturing conditions, ectopic expression of Samp1 induced a rapid differentiation of iPSCs, ofwhich some expressed the neuronal marker beta III-tubulin already after 6 days. This suggests that Samp1 is involved in early differentiation of iPSCs and could potentially be explored as a tool to promote progression of the differentiation process.

Place, publisher, year, edition, pages
2017. Vol. 23, p. 33-38
Keywords [en]
Nuclear membrane, Nuclear envelope, Induced pluripotent stem cells, Neuronal differentiation, Regenerative medicine
National Category
Medical Engineering Environmental Biotechnology Cell Biology
Identifiers
URN: urn:nbn:se:su:diva-147935DOI: 10.1016/j.scr.2017.06.008ISI: 000410958100004PubMedID: 28668644OAI: oai:DiVA.org:su-147935DiVA, id: diva2:1149994
Available from: 2017-10-17 Created: 2017-10-17 Last updated: 2017-10-17Bibliographically approved

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Bergqvist, CeciliaJafferali, Mohammed HakimHallberg, Einar
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