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MCLIP Detection of Novel Protein-Protein Interactions at the Nuclear Envelope
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Neurochemistry.
Number of Authors: 3
2016 (English)In: Intermediate Filament Associated Proteins / [ed] Katherine L. Wilson, Arnoud Sonnenberg, SAN DIEGO: ELSEVIER ACADEMIC PRESS INC , 2016, Vol. 569, 503-515 p.Chapter in book (Refereed)
Abstract [en]

The organization and function of the nuclear envelope (NE) involves hundreds of nuclear membrane proteins and myriad protein-protein interactions, most of which are still uncharacterized. Many NE proteins interact stably or dynamically with the nuclear lamina or chromosomes. This can make them difficult to extract under non-denaturing conditions, and greatly limits our ability to explore and identify functional protein interactions at the NE. This knowledge is needed to understand nuclear envelope structure and the mechanisms of human laminopathy diseases. This chapter provides detailed protocols for MCLIP (membrane cross-linking immunoprecipitation) identification of novel protein-protein interactions in mammalian cells.

Place, publisher, year, edition, pages
SAN DIEGO: ELSEVIER ACADEMIC PRESS INC , 2016. Vol. 569, 503-515 p.
Series
Methods in Enzymology, ISSN 0076-6879 ; 569
Keyword [en]
Nucleus, Nuclear envelope, Nuclear lamina, Nuclear membrane, Protein–protein interactions, Proteomics, MCLIP
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-148114DOI: 10.1016/bs.mie.2015.08.022ISI: 000410546200025PubMedID: 26778573ISBN: 978-0-12-803469-9 (print)ISBN: 0-12-803469-6 (print)OAI: oai:DiVA.org:su-148114DiVA: diva2:1150744
Available from: 2017-10-19 Created: 2017-10-19 Last updated: 2017-10-19Bibliographically approved

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