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Structures of the human mitochondrial ribosome in native states of assembly
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
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Number of Authors: 82017 (English)In: Nature Structural & Molecular Biology, ISSN 1545-9993, E-ISSN 1545-9985, Vol. 24, no 10, p. 866-869Article in journal (Refereed) Published
Abstract [en]

Mammalian mitochondrial ribosomes (mitoribosomes) have less rRNA content and 36 additional proteins compared with the evolutionarily related bacterial ribosome. These differences make the assembly of mitoribosomes more complex than the assembly of bacterial ribosomes, but the molecular details of mitoribosomal biogenesis remain elusive. Here, we report the structures of two late-stage assembly intermediates of the human mitoribosomal large subunit (mt-LSU) isolated from a native pool within a human cell line and solved by cryo-EM to similar to 3-angstrom resolution. Comparison of the structures reveals insights into the timing of rRNA folding and protein incorporation during the final steps of ribosomal maturation and the evolutionary adaptations that are required to preserve biogenesis after the structural diversification of mitoribosomes. Furthermore, the structures redefine the ribosome silencing factor (RsfS) family as multifunctional biogenesis factors and identify two new assembly factors (L0R8F8 and mt-ACP) not previously implicated in mitoribosomal biogenesis.

Place, publisher, year, edition, pages
2017. Vol. 24, no 10, p. 866-869
Keywords [en]
Cryoelectron microscopy, Ribosome
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-148876DOI: 10.1038/nsmb.3464ISI: 000412278000014PubMedID: 28892042OAI: oai:DiVA.org:su-148876DiVA, id: diva2:1159201
Available from: 2017-11-22 Created: 2017-11-22 Last updated: 2017-11-22Bibliographically approved

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Rathore, SorbhiKimanius, DariAibara, ShintaroAmunts, Alexey
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