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Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H
Stockholm University, Faculty of Science, Department of Organic Chemistry.
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Number of Authors: 13
2017 (English)In: Antonie van Leeuwenhoek. International Journal of General and Molecular Microbiology, ISSN 0003-6072, E-ISSN 1572-9699, Vol. 110, no 11, 1377-1387 p.Article in journal (Refereed) Published
Abstract [en]

Colwellia psychrerythraea strain 34H, a Gram-negative bacterium isolated from Arctic marine sediments, is considered a model to study the adaptation to cold environments. Recently, we demonstrated that C. psychrerythraea 34H produces two different extracellular polysaccharides, a capsular polysaccharide and a medium released polysaccharide, which confer cryoprotection to the bacterium. In this study, we report the structure of an additional capsular polysaccharide produced by Colwellia grown at a different temperature. The structure was determined using chemical methods, and one- and two-dimensional NMR spectroscopy. The results showed a trisaccharide repeating unit made up of only amino-sugar residues: N-acetyl-galactosamine, 2,4-diacetamido-2,4,6-trideoxy-glucose (bacillosamine), and 2-acetamido-2-deoxyglucuronic acid with the following structure: -> 4)-beta-d-GlcpNAcA-(1 -> 3)-beta-d-QuipNAc4NAc-(1 -> 3)-beta-d-GalpNAc-(1 ->. The 3D model, generated in accordance with H-1,H-1-NOE NMR correlations and consisting of ten repeating units, shows a helical structure. In contrast with the other extracellular polysaccharides produced from Colwellia at 4 A degrees C, this molecule displays only a low ice recrystallization inhibition activity.

Place, publisher, year, edition, pages
2017. Vol. 110, no 11, 1377-1387 p.
Keyword [en]
Cold adaptation, Extracellular polysaccharides, NMR, Psychrophile, Anti-freeze activity
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:su:diva-148959DOI: 10.1007/s10482-017-0834-6ISI: 000413219700002PubMedID: 28161737OAI: oai:DiVA.org:su-148959DiVA: diva2:1163818
Conference
7th Biennial Baltic Meeting on Microbial Carbohydrates (BMMC), Gustrow, Germany, September 25-29, 2016
Available from: 2017-12-08 Created: 2017-12-08 Last updated: 2017-12-08Bibliographically approved

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Ståhle, JonasWidmalm, GöranTutino, Maria L.
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