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CBP Regulates Recruitment and Release of Promoter-Proximal RNA Polymerase II
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
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Number of Authors: 10
2017 (English)In: Molecular Cell, ISSN 1097-2765, E-ISSN 1097-4164, Vol. 68, no 3, 491-+ p.Article in journal (Refereed) Published
Abstract [en]

Transcription activation involves RNA polymerase II (Pol II) recruitment and release from the promoter into productive elongation, but how specific chromatin regulators control these steps is unclear. Here, we identify a novel activity of the histone acetyltransferase p300/CREB-binding protein (CBP) in regulating promoter-proximal paused Pol II. We find that Drosophila CBP inhibition results in dribbling'' of Pol II from the pause site to positions further downstream but impedes transcription through the + 1 nucleosome genome-wide. Promoters strongly occupied by CBP and GAGA factor have high levels of paused Pol II, a unique chromatin signature, and are highly expressed regardless of cell type. Interestingly, CBP activity is rate limiting for Pol II recruitment to these highly paused promoters through an interaction with TFIIB but for transit into elongation by histone acetylation at other genes. Thus, CBP directly stimulates both Pol II recruitment and the ability to traverse the first nucleosome, thereby promoting transcription of most genes.

Place, publisher, year, edition, pages
2017. Vol. 68, no 3, 491-+ p.
National Category
Biological Sciences
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URN: urn:nbn:se:su:diva-149993DOI: 10.1016/j.molcel.2017.09.031ISI: 000414250700005PubMedID: 29056321OAI: oai:DiVA.org:su-149993DiVA: diva2:1168718
Available from: 2017-12-21 Created: 2017-12-21 Last updated: 2017-12-21Bibliographically approved

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Boija, AnnMannervik, Mattias
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