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SRP, FtsY, DnaK and YidC Are Required for the Biogenesis of the E. coli Tail-Anchored Membrane Proteins DjIC and Flk
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
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Number of Authors: 7
2018 (English)In: Journal of Molecular Biology, ISSN 0022-2836, E-ISSN 1089-8638, Vol. 430, no 3, p. 389-403Article in journal (Refereed) Published
Abstract [en]

Tail-anchored membrane proteins (TAMPs) are relatively simple membrane proteins characterized by a single transmembrane domain (TMD) at their C-terminus. Consequently, the hydrophobic TMD, which acts as a subcellular targeting signal, emerges from the ribosome only after termination of translation precluding canonical co-translational targeting and membrane insertion. In contrast to the well-studied eukaryotic TAMPs, surprisingly little is known about the cellular components that facilitate the biogenesis of bacterial TAMPs. In this study, we identify DjIC and Flk as bona fide Escherichia co/iTAMPs and show that their TMDs are necessary and sufficient for authentic membrane targeting of the fluorescent reporter mNeonGreen. Using strains conditional for the expression of known E. coli membrane targeting and insertion factors, we demonstrate that the signal recognition particle (SRP), its receptor FtsY, the chaperone DnaK and insertase YidC are each required for efficient membrane localization of both TAMPs. A close association between the TMD of DjIC and Flk with both the Ffh subunit of SRP and YidC was confirmed by site-directed in vivo photo-crosslinking. In addition, our data suggest that the hydrophobicity of the TMD correlates with the dependency on SRP for efficient targeting.

Place, publisher, year, edition, pages
2018. Vol. 430, no 3, p. 389-403
Keyword [en]
membrane targeting, membrane insertion, membrane protein, tail-anchored, E. coli
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-153623DOI: 10.1016/j.jmb.2017.12.004ISI: 000424961400012PubMedID: 29246766OAI: oai:DiVA.org:su-153623DiVA, id: diva2:1189983
Available from: 2018-03-13 Created: 2018-03-13 Last updated: 2018-03-13Bibliographically approved

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Karyolaimos, Alexandrosde Gier, Jan-Willem
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