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MonoRes: Automatic and Accurate Estimation of Local Resolution for Electron Microscopy Maps
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Number of Authors: 112018 (English)In: Structure, ISSN 0969-2126, E-ISSN 1878-4186, Vol. 26, no 2, p. 337-344Article in journal (Refereed) Published
Abstract [en]

Since the beginning of electron microscopy, resolution has been a critical parameter. In this article, we propose a fully automatic, accurate method for determining the local resolution of a 3D map (MonoRes). The foundation of this algorithm is an extension of the concept of analytic signal, termed monogenic signal. The map is filtered at different frequencies and the amplitude of the monogenic signal is calculated, after which a criterion is applied to determine the resolution at each voxel. MonoRes is fully automatic without compulsory user parameters, with great accuracy in all tests, and is computationally more rapid than existing methods in the field. In addition, MonoRes offers the option of local filtering of the original map based on the calculated local resolution.

Place, publisher, year, edition, pages
2018. Vol. 26, no 2, p. 337-344
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-153775DOI: 10.1016/j.str.2017.12.018ISI: 000424806800017PubMedID: 29395788OAI: oai:DiVA.org:su-153775DiVA, id: diva2:1192351
Available from: 2018-03-22 Created: 2018-03-22 Last updated: 2018-03-22Bibliographically approved

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Miguel de la Rosa-Trevín, José
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Department of Biochemistry and BiophysicsScience for Life Laboratory (SciLifeLab)
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