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Efficient DNA-assisted synthesis of trans-membrane gold nanowires
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
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Number of Authors: 5
2018 (English)In: microsystems and nanoengineering, ISSN 2055-7434, Vol. 4, article id UNSP 17084Article in journal (Refereed) Published
Abstract [en]

Whereas electric circuits and surface-based (bio) chemical sensors are mostly constructed in-plane due to ease of manufacturing, 3D microscale and nanoscale structures allow denser integration of electronic components and improved mass transport of the analyte to (bio) chemical sensor surfaces. This work reports the first out-of-plane metallic nanowire formation based on stretching of DNA through a porous membrane. We use rolling circle amplification (RCA) to generate long single-stranded DNA concatemers with one end anchored to the surface. The DNA strands are stretched through the pores in the membrane during liquid removal by forced convection. Because the liquid-air interface movement across the membrane occurs in every pore, DNA stretching across the membrane is highly efficient. The stretched DNA molecules are transformed into trans-membrane gold nanowires through gold nanoparticle hybridization and gold enhancement chemistry. A 50 fM oligonucleotide concentration, a value two orders of magnitude lower than previously reported for flat surface-based nanowire formation, was sufficient for nanowire formation. We observed nanowires in up to 2.7% of the membrane pores, leading to an across-membrane electrical conductivity reduction from open circuit to <20 Omega. The simple electrical read-out offers a high signal-to-noise ratio and can also be extended for use as a biosensor due to the high specificity and scope for multiplexing offered by RCA.

Place, publisher, year, edition, pages
2018. Vol. 4, article id UNSP 17084
Keyword [en]
DNA stretching, DNA detection, gold nanowires, nanowire synthesis, membrane
National Category
Chemical Sciences Nano Technology
Identifiers
URN: urn:nbn:se:su:diva-154594DOI: 10.1038/micronano.2017.84ISI: 000425451300001OAI: oai:DiVA.org:su-154594DiVA, id: diva2:1195398
Available from: 2018-04-05 Created: 2018-04-05 Last updated: 2018-04-05Bibliographically approved

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Hernández-Neuta, IvánNilsson, Mats
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