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NMR Study of Rcf2 Reveals an Unusual Dimeric Topology in Detergent Micelles
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
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Number of Authors: 52018 (English)In: ChemBioChem (Print), ISSN 1439-4227, E-ISSN 1439-7633, Vol. 19, no 5, p. 444-447Article in journal (Refereed) Published
Abstract [en]

The Saccharomyces cerevisiae mitochondrial respiratory supercomplex factor2 (Rcf2) plays a role in assembly of supercomplexes composed of cytochromebc(1) (complexIII) and cytochromec oxidase (complexIV). We expressed the Rcf2 protein in Escherichia coli, refolded it, and reconstituted it into dodecylphosphocholine (DPC) micelles. The structural properties of Rcf2 were studied by solution NMR, and near complete backbone assignment of Rcf2 was achieved. The secondary structure of Rcf2 contains seven helices, of which five are putative transmembrane (TM) helices, including, unexpectedly, a region formed by a charged 20-residue helix at the Cterminus. Further studies demonstrated that Rcf2 forms a dimer, and the charged TM helix is involved in this dimer formation. Our results provide a basis for understanding the role of this assembly/regulatory factor in supercomplex formation and function.

Place, publisher, year, edition, pages
2018. Vol. 19, no 5, p. 444-447
Keywords [en]
dimers, membrane protein, membrane topology, NMR spectroscopy, respiratory supercomplex factor
National Category
Biological Sciences Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-154787DOI: 10.1002/cbic.201700664ISI: 000426494600009PubMedID: 29240987OAI: oai:DiVA.org:su-154787DiVA, id: diva2:1198472
Available from: 2018-04-17 Created: 2018-04-17 Last updated: 2018-04-17Bibliographically approved

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Zhou, ShuPettersson, PontusBrzezinski, PeterÄdelroth, PiaMäler, Lena
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