Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Cell-penetrating peptides for siRNA delivery to glioblastomas
Stockholm University, Faculty of Science, Department of Neurochemistry.ORCID iD: 0000-0003-2265-557X
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Neurochemistry.
Show others and affiliations
Number of Authors: 52018 (English)In: Peptides, ISSN 0196-9781, E-ISSN 1873-5169, Vol. 104, p. 62-69Article in journal (Refereed) Published
Abstract [en]

Delivery of small interfering RNA (siRNA) to suppress glioblastoma growth is a hurdle due to the critical obstacles of the blood-brain barrier and the siRNA properties of such as high negative charges and instability in serum. Therefore, the passage of siRNA to targeted cells is limited. Several siRNA carriers have been constructed using cell-penetrating peptides (CPPs) since the CPPs have shown a high potential for oligonucleotide delivery into the cells. In this study, two CPPs, PepFect 14 (PF14) and the amphipathic peptide PepFect 28 (PF28), were modified with targeting peptides by covalent conjugation and non-covalent complex formation to improve glioma-targeted specificity and gene-silencing efficiency. In conclusion, we have established an efficient non-covalently complexed carrier (PF14:TG1) for siRNA delivery to human glioblastoma cells (U87), showing a significant two-fold increase in gene-silencing efficiency compared to the parent peptide PF14 and also improved specificity to U87 cells compared to non-glioma targeted cells.

Place, publisher, year, edition, pages
2018. Vol. 104, p. 62-69
Keywords [en]
Glioblastomas, Cell-penetrating peptide, siRNA, Glioma-targeted drug delivery, Targeting peptide, Angiopep-2, PepFect
National Category
Biological Sciences Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-157713DOI: 10.1016/j.peptides.2018.04.015ISI: 000432591600008PubMedID: 29684592OAI: oai:DiVA.org:su-157713DiVA, id: diva2:1236213
Available from: 2018-08-01 Created: 2018-08-01 Last updated: 2018-08-01Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Srimanee, ArtitaHällbrink, MattiasLangel, Ülo
By organisation
Department of Neurochemistry
In the same journal
Peptides
Biological SciencesCell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf