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SWI/SNF regulates half of its targets without the need of ATP-driven nucleosome remodeling by Brahma
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
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Number of Authors: 62018 (English)In: BMC Genomics, ISSN 1471-2164, E-ISSN 1471-2164, Vol. 19, article id 367Article in journal (Refereed) Published
Abstract [en]

Background: Brahma (BRM) is the only catalytic subunit of the SVVI/SNF chromatin-remodeling complex of Drosophila melanogaster. The function of SWI/SNF in transcription has long been attributed to its ability to remodel nucleosomes, which requires the ATPase activity of BRM. However, recent studies have provided evidence for a non-catalytic function of BRM in the transcriptional regulation of a few specific genes.

Results: Here we have used RNA-seq and ChIP-seq to identify the BRM target genes in 52 cells, and we have used a catalytically inactive BRM mutant (K804R) that is unable to hydrolyze ATP to investigate the magnitude of the non-catalytic function of BRM in transcription regulation. We show that 49% of the BRM target genes in 52 cells are regulated through mechanisms that do not require BRM to have an ATPase activity. We also show that the catalytic and non-catalytic mechanisms of SVVI/SNF regulation operate on two subsets of genes that differ in promoter architecture and are linked to different biological processes.

Conclusions: This study shows that the non-catalytic role of SWI/SNF in transcription regulation is far more prevalent than previously anticipated and that the genes that are regulated by SVVI/SNF through ATPase-dependent and ATPase-independent mechanisms have specialized roles in different cellular and developmental processes.

Place, publisher, year, edition, pages
2018. Vol. 19, article id 367
Keywords [en]
SWI/SNF, Gene expression, Transcription regulation, Drosophila melanogaster
National Category
Environmental Biotechnology Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-157741DOI: 10.1186/s12864-018-4746-2ISI: 000432702400002PubMedID: 29776334OAI: oai:DiVA.org:su-157741DiVA, id: diva2:1236658
Available from: 2018-08-03 Created: 2018-08-03 Last updated: 2018-08-03Bibliographically approved

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Jordán-Pla, AntonioÖstlund Farrants, Ann-KristinVisa, Neus
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