Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Drosophila insulin-like peptide 1 (DILP1) promotes organismal growth during non-feeding stages
Stockholm University, Faculty of Science, Department of Zoology.ORCID iD: 0000-0003-2828-6891
Show others and affiliations
(English)Manuscript (preprint) (Other academic)
National Category
Biological Sciences
Research subject
Functional Zoomorphology
Identifiers
URN: urn:nbn:se:su:diva-159977OAI: oai:DiVA.org:su-159977DiVA, id: diva2:1247552
Available from: 2018-09-12 Created: 2018-09-12 Last updated: 2019-12-10Bibliographically approved
In thesis
1. The role of insulin signaling during development, reproductive diapause and aging in Drosophila Melanogaster
Open this publication in new window or tab >>The role of insulin signaling during development, reproductive diapause and aging in Drosophila Melanogaster
2018 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The insulin/insulin-like growth factor signaling pathway exists from invertebrates to vertebrates and it can regulate various biological processes, including development, metabolism, stress resistance and lifespan. In Drosophila, eight insulin-like peptides (DILP1-8) have been found. The specific function of each DILP is not fully known, especially for DILP1. In paper I, we found that dilp1 is specifically expressed in the brain insulin producing cells (IPCs), and it is mainly expressed from early pupa until few days of adult life, which correspond to non-feeding stages. The expression of dilp1 can last for at least 9 weeks of adult life when newborn virgin flies are induced to enter reproductive diapause. In addition, we found that the expression of dilp1 is under regulation by other dilps. Also larva-derived fat body, short neuropeptide F (sNPF) and juvenile hormone can affect dilp1 expression. We found that mutation of dilp1 affects female reproduction and starvation resistance. In paper II, we found that reproductive diapause can extend Drosophila life span, and at the same time ameliorate behavioral senescence, including negative geotaxis, activity rhythms and exploratory walking. Age-related changes in neuromuscular junction (NMJ) in abdominal muscle cannot be found in diapause-induced aging flies. The levels of several neuromodulators in the brain, including pigment dispersion factor (PDF), tyrosine hydroxylase (TH) and short neuropeptide F (sNPF), decreased significantly in normally aging flies, but less so in diapausing flies. In paper III, we show that mutation of dilp1 leads to a reduced organismal bodyweight, whereas overexpression increases it during the nonfeeding pupal stage. Overexpression of dilp1 additionally increases body size of flies, but reduces stores of larval-derived energy. This results in decreased starvation tolerance and increased feeding in newborn flies. In paper IV, we found that dilp1 expression is needed to extend lifespan in dilp2 mutant flies. Single dilp1 mutation has no effect on female lifespan, whereas transgene expression of dilp1 in flies with dilp1-dilp2 double mutant genetic background increased the lifespan. Furthermore, dilp1 and dilp2 interact to control circulating sugar, starvation resistance in a redundant or synergistic way.

Place, publisher, year, edition, pages
Stockholm: Department of Zoology, Stockholm University, 2018
Keywords
insulin-like peptides, reproductive diapause, behavioral senescence, neuromodulator, lifespan
National Category
Zoology
Research subject
Functional Zoomorphology
Identifiers
urn:nbn:se:su:diva-159978 (URN)978-91-7797-431-4 (ISBN)978-91-7797-430-7 (ISBN)
Public defence
2018-11-02, Vivi Täckholmssalen (Q-salen), NPQ-huset, Svante Arrhenius Väg 20, Stockholm, 13:00 (English)
Opponent
Supervisors
Funder
Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning, VR: 621-2010-5742 and 2015-04626
Available from: 2018-10-10 Created: 2018-09-13 Last updated: 2020-05-11Bibliographically approved

Open Access in DiVA

No full text in DiVA

Search in DiVA

By author/editor
Liao, SifangNässel, Dick
By organisation
Department of Zoology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 41 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf