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Shaping Escherichia coli for recombinant membrane protein production
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Number of Authors: 42018 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 365, no 15, article id fny152Article, review/survey (Refereed) Published
Abstract [en]

The bacterium Escherichia coli has been widely used for the production of both pro- and eukaryotic membrane proteins. Usually, a set of standard strains as well as different culture setups and induction regimes are screened for to enhance production yields. However, on a limited scale, E. coli strains have been isolated for recombinant helical bundle membrane protein production using both selection- and engineering-based approaches. Here, we discuss how such approaches have been used so far to shape E. coli for the production of these recombinant membrane proteins and may be used in the future to further enhance production yields.

Place, publisher, year, edition, pages
2018. Vol. 365, no 15, article id fny152
Keywords [en]
Escherichia coli, membrane protein, protein production, selection, screen, strain engineering
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-160258DOI: 10.1093/femsle/fny152ISI: 000441115500002PubMedID: 30007322OAI: oai:DiVA.org:su-160258DiVA, id: diva2:1249543
Available from: 2018-09-19 Created: 2018-09-19 Last updated: 2018-09-19Bibliographically approved

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Karyolaimos, AlexandrosAmpah-Korsah, HenryZhang, Zhede Gier, Jan-Willem
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