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Flow Cytometry of Mouse and Human Adipocytes for the Analysis of Browning and Cellular Heterogeneity
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Number of Authors: 132018 (English)In: Cell reports, ISSN 2211-1247, E-ISSN 2211-1247, Vol. 24, no 10, p. 2746-2756Article in journal (Refereed) Published
Abstract [en]

Adipocytes, once considered simple lipid-storing cells, are rapidly emerging as complex cells with many biologically diverse functions. A powerful high-throughput method for analyzing single cells is flow cytometry. Several groups have attempted to analyze and sort freshly isolated adipocytes; however, using an adipocyte-specific reporter mouse, we demonstrate that these studies fail to detect the majority of white adipocytes. We define critical settings required for adipocyte flow cytometry and provide a rigid strategy for analyzing and sorting white and brown adipocyte populations. The applicability of our protocol is shown by sorting mouse adipocytes based on size or UCP1 expression and demonstrating that a subset of human adipocytes lacks the beta(2)-adrenergic receptor, particularly in the insulin-resistant state. In conclusion, the present study confers key technological insights for analyzing and sorting mature adipocytes, opening up numerous downstream research applications.

Place, publisher, year, edition, pages
2018. Vol. 24, no 10, p. 2746-2756
Keywords [en]
adipocyte, adipose tissue, flow cytometry, FACS, mouse, human, uncoupled protein 1, beta 2 adrenergic receptor
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-160206DOI: 10.1016/j.celrep.2018.08.006ISI: 000443588800021PubMedID: 30184507OAI: oai:DiVA.org:su-160206DiVA, id: diva2:1251101
Available from: 2018-09-26 Created: 2018-09-26 Last updated: 2018-09-26Bibliographically approved

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Shabalina, Irina G.Nedergaard, Jan
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Department of Molecular Biosciences, The Wenner-Gren Institute
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