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An engineered thermal-shift screen reveals specific lipid preferences of eukaryotic and prokaryotic membrane proteins
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Number of Authors: 42018 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 9, article id 4253Article in journal (Refereed) Published
Abstract [en]

Membrane bilayers are made up of a myriad of different lipids that regulate the functional activity, stability, and oligomerization of many membrane proteins. Despite their importance, screening the structural and functional impact of lipid-protein interactions to identify specific lipid requirements remains a major challenge. Here, we use the FSEC-TS assay to show cardiolipin-dependent stabilization of the dimeric sodium/proton antiporter NhaA, demonstrating its ability to detect specific protein-lipid interactions. Based on the principle of FSECTS, we then engineer a simple thermal-shift assay (GFP-TS), which facilitates the highthroughput screening of lipid-and ligand-interactions with membrane proteins. By comparing the thermostability of medically relevant eukaryotic membrane proteins and a selection of bacterial counterparts, we reveal that eukaryotic proteins appear to have evolved to be more dependent to the presence of specific lipids.

Place, publisher, year, edition, pages
2018. Vol. 9, article id 4253
National Category
Biochemistry and Molecular Biology Biophysics
Identifiers
URN: urn:nbn:se:su:diva-161947DOI: 10.1038/s41467-018-06702-3ISI: 000447123000029PubMedID: 30315156OAI: oai:DiVA.org:su-161947DiVA, id: diva2:1262550
Available from: 2018-11-12 Created: 2018-11-12 Last updated: 2018-11-12Bibliographically approved

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