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In Mitochondria beta-Actin Regulates mtDNA Transcription and Is Required for Mitochondrial Quality Control
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute. New York University Abu Dhabi (NYUAD), United Arab Emirates.
Number of Authors: 42018 (English)In: iScience, ISSN 2589-0042, Vol. 3, p. 226-237Article in journal (Refereed) Published
Abstract [en]

In eukaryotic cells, actin regulates both cytoplasmic and nuclear functions. However, whether actin-based structures are present in the mitochondria and are involved in mitochondrial functions has not been investigated. Here, using wild-type beta-actin +/+ and knockout (KO) b-actin -/- mouse embryonic fibroblasts we show evidence for the defect in maintaining mitochondrial membrane potential (MMP) in beta-actin-null cells. MMP defects were associated with impaired mitochondrial DNA (mtDNA) transcription and nuclear oxidative phosphorylation (OXPHOS) gene expression. Using super-resolution microscopy we provided direct evidence on the presence of beta-actin-containing structures inside mitochondria. Large aggregates of TFAM-stained nucleoids were observed in bulb-shaped mitochondria in KO cells, suggesting defects in mitochondrial nucleoid segregation without beta-actin. The observation that mitochondria-targeted beta-actin rescued mtDNA transcription and MMP suggests an indispensable functional role of a mitochondrial beta-actin pool necessary for mitochondrial quality control.

Place, publisher, year, edition, pages
2018. Vol. 3, p. 226-237
National Category
Other Natural Sciences
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URN: urn:nbn:se:su:diva-162959DOI: 10.1016/j.isci.2018.04.021ISI: 000449721600019PubMedID: 30428323OAI: oai:DiVA.org:su-162959DiVA, id: diva2:1270104
Available from: 2018-12-12 Created: 2018-12-12 Last updated: 2018-12-12Bibliographically approved

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Percipalle, Piergiorgio
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