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Structure of a functional obligate complex III2IV2 respiratory supercomplex from Mycobacterium smegmatis
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.ORCID iD: 0000-0002-2994-5839
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
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Number of Authors: 122018 (English)In: Nature Structural & Molecular Biology, ISSN 1545-9993, E-ISSN 1545-9985, Vol. 25, no 12, p. 1128-1136Article in journal (Refereed) Published
Abstract [en]

In the mycobacterial electron-transport chain, respiratory complex III passes electrons from menaquinol to complex IV, which in turn reduces oxygen, the terminal acceptor. Electron transfer is coupled to transmembrane proton translocation, thus establishing the electrochemical proton gradient that drives ATP synthesis. We isolated, biochemically characterized, and determined the structure of the obligate III2IV2 supercomplex from Mycobacterium smegmatis, a model for Mycobacterium tuberculosis. The supercomplex has quinol:O-2 oxidoreductase activity without exogenous cytochrome c and includes a superoxide dismutase subunit that may detoxify reactive oxygen species produced during respiration. We found menaquinone bound in both the Q(o) and Q(i) sites of complex III. The complex III-intrinsic diheme cytochrome cc subunit, which functionally replaces both cytochrome c(1) and soluble cytochrome c in canonical electron-transport chains, displays two conformations: one in which it provides a direct electronic link to complex IV and another in which it serves as an electrical switch interrupting the connection.

Place, publisher, year, edition, pages
2018. Vol. 25, no 12, p. 1128-1136
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Biological Sciences
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URN: urn:nbn:se:su:diva-163582DOI: 10.1038/s41594-018-0160-3ISI: 000452543300013PubMedID: 30518849OAI: oai:DiVA.org:su-163582DiVA, id: diva2:1278445
Available from: 2019-01-14 Created: 2019-01-14 Last updated: 2019-01-14Bibliographically approved

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Wiseman, BenjaminNitharwal, Ram GopalFedotovskaya, OlgaSchäfer, JacobGuo, HuiKuang, QieSjöstrand, DanÄdelroth, PiaRubinstein, John L.Brzezinski, PeterHögbom, Martin
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