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The MEMbrane Protein Single ShoT Amplification Recipe: MemStar
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Number of Authors: 42017 (English)In: A Structure-Function Toolbox for Membrane Transporter and Channels / [ed] Christine Ziegler, San Diego: Elsevier, 2017, Vol. 594, p. 123-138Chapter in book (Refereed)
Abstract [en]

Here, we present a simple overexpression condition for high-throughput screening of membrane proteins in Escherichia coli. For the vast majority of bacterial membrane protein targets tested the MEMbrane protein Single shoT Amplification Recipe-MemStarleads to high production yields of target protein. The use of MemStar has facilitated structural studies of several transport proteins.

Place, publisher, year, edition, pages
San Diego: Elsevier, 2017. Vol. 594, p. 123-138
Series
Methods in Enzymology, ISSN 0076-6879 ; 594
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-163631DOI: 10.1016/bs.mie.2017.05.010ISI: 000452356400006PubMedID: 28779838ISBN: 978-0-12-812353-9 (print)OAI: oai:DiVA.org:su-163631DiVA, id: diva2:1282085
Available from: 2019-01-24 Created: 2019-01-24 Last updated: 2019-01-24Bibliographically approved

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Aziz-Qureshi, AbdulMeier, Pascal F.Drew, David
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