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Analysis of butterfly reproductive proteins using capillary electrophoresis and mass spectrometry
Stockholm University, Faculty of Science, Department of Zoology.
Number of Authors: 42019 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 566, p. 23-26Article in journal (Refereed) Published
Abstract [en]

A method for analysis of proteins from spermatophores transferred from male to female Pieris napi butterflies during mating has been developed. The proteins were solubilized from the dissected spermatophores using different solubilization agents (water, methanol, acetonitrile and hexafluoroisopropanol). Capillary electrophoresis (CE) analysis was performed using an acidic background electrolyte containing a fluorosurfactant to avoid protein-wall adsorption, and to increase separation performance. The samples were also analyzed with matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), in a lower m/z range (1000-6000) and a higher m/z range (6000-12000). Solubilization with different solvents and the use of alternative matrices gave partly complementary profiles.

Place, publisher, year, edition, pages
2019. Vol. 566, p. 23-26
Keywords [en]
Capillary electrophoresis, Matrix assisted laser desorption/ionization, mass spectrometry, Pieris napi, Seminal fluid proteins, Spermatophore
National Category
Biological Sciences Chemical Sciences
Identifiers
URN: urn:nbn:se:su:diva-165633DOI: 10.1016/j.ab.2018.11.002ISI: 000456354900006PubMedID: 30423321OAI: oai:DiVA.org:su-165633DiVA, id: diva2:1287058
Available from: 2019-02-08 Created: 2019-02-08 Last updated: 2019-02-08Bibliographically approved

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