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Cyanobacteria as a model system for studies of oxygenic photosynthesis and respiration
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
1992 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Virtually all the energy available to living organisms is derived from solar radiation. The light energy from the sun is harvested by plants, algae and phototrophic bacteria and converted to chemical energy through the process of photosynthesis.

Cyanobacteria are procaryotic organisms capable of oxygenic photosynthesis. They are thought to be the first organisms to have developed photosystem II and the ability to split water and they are also capable of energy conversion through respiration. The chloroplast of higher plants displays many similarities to cyanobacteria, both functionally and structurally, and is thought to have originated from a primitive cyanobacteria.

As procaryotes, cyanobacteria are excellent organisms for studies of oxygenic photosynthesis and have been widely used for constructing mutants with deleted or altered genes coding for central components of the photosynthetic apparatus. Such mutants have been utilized to study both structural and functional aspects of photosystem II.

The lack of adequate procedures for biochemical handling and subfractionation of cyanobacteria has however limited the usefulness of this approach, since it has not been possible to fully characterize these mutants using biochemical and biophysical techniques.

To overcome this, preparative procedures were developed for isolation of different cyanobacterial membranes. Photosystem II enriched thylakoid membranes were prepared and highly active photosystem II membranes were isolated from the transformable cyanobacterium Synechocystis 6803 by selective detergent extraction of photosystem I. This preparation is highly suited for EPR spectroscopy. Cyanobacterial plasma membranes and everted thylakoid vesicles were isolated by aqueoues polymer two-phase partitioning. These preparative procedures contributes to the development of cyanobacteria as a powerful tool for studies on photosynthesis and respiration.

The potential of combining molecular techniques, such as the construction of mutants, with detailed biochemical characterization was demonstrated. Using this approach, important conclusions could be reached concerning excitation energy transfer, protein stability, photosystem synthesis and organization in cyanobacteria. A photosystem II subcomplex was detected in a mutant lacking intact reaction centers, and based on this finding model for the assembly of photosystem II is proposed.

Place, publisher, year, edition, pages
Stockholm: Stockholm University, 1992. , p. 43
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-166043Libris ID: 7609010ISBN: 91-7146-991-5 (print)OAI: oai:DiVA.org:su-166043DiVA, id: diva2:1288041
Public defence
1992-04-15, Hörsal G, Arrheniuslaboratorierna, Svante Arrhenius väg 18, Stockholm, 10:00
Note

Härtill 5 uppsatser

Available from: 2019-02-12 Created: 2019-02-12 Last updated: 2019-02-12Bibliographically approved

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