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Determination of the small amount of proteins interacting with TiO2 nanotubes by AFM-measurement
Stockholm University, Faculty of Science, Department of Materials and Environmental Chemistry (MMK). Nanjing Tech University, China; Uppsala University, Sweden; Petru Poni Institute of Macromolecular Chemistry, Romania.
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Number of Authors: 72019 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 192, p. 368-376Article in journal (Refereed) Published
Abstract [en]

Detecting the small amounts of proteins interacting effectively with the solid film electrodes surface still remains a challenge. To address this, in this work, a new approach was proposed by the combination of the adhesion forces and the molecular interaction measured with AFM. Cytochrome c (Cyt C) interacting effectively with TiO2 nanotube arrays (TNAs) was chosen as a probe. The amounts of Cyt C molecules interacting effectively on TNAs surface (C-TNA) range from 5.5x10(-12) to 7.0x10(-12) mol/cm(2) (68.2-86.8 ng/cm(2)) and they are comparable with the values obtained by the electrochemistry method in the literature, in evidence of the accuracy of this AFM-based approach. The reliability of the proposed approach was further verified by conducting Surface Enhanced Raman Scattering (SERS) measurements and estimating the enhancement factor (EF). This interaction-based AFM approach can be used to accurately obtain the small amounts of adsorbed substances on the solid film electrodes surface in the applications such as biosensors, biocatalysis, and drug delivery, etc.

Place, publisher, year, edition, pages
2019. Vol. 192, p. 368-376
Keywords [en]
TiO2 nanotubes, Adsorption amount, AFM, Molecular interaction, Adhesion force, SERS
National Category
Medical Engineering Industrial Biotechnology
Identifiers
URN: urn:nbn:se:su:diva-166770DOI: 10.1016/j.biomaterials.2018.11.013ISI: 000456902000030PubMedID: 30476718OAI: oai:DiVA.org:su-166770DiVA, id: diva2:1295988
Available from: 2019-03-13 Created: 2019-03-13 Last updated: 2019-03-13Bibliographically approved

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