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Oxidative hotspots on actin promote skeletal muscle weakness in rheumatoid arthritis
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
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Number of Authors: 232019 (English)In: JCI Insight, ISSN 2379-3708, Vol. 4, no 9, article id e126347Article in journal (Refereed) Published
Abstract [en]

Skeletal muscle weakness in patients suffering from rheumatoid arthritis (RA) adds to their impaired working abilities and reduced quality of life. However, little molecular insight is available on muscle weakness associated with RA. Oxidative stress has been implicated in the disease pathogenesis of RA. Here, we show that oxidative posttranslational modifications of the contractile machinery targeted to actin result in impaired actin polymerization and reduced force production. Using mass spectrometry, we identified the actin residues targeted by oxidative 3-nitrotyrosine (3-NT) or malondialdehyde (MDA) adduct modifications in weakened skeletal muscle from mice with arthritis and patients afflicted by RA. The residues were primarily located in 3 distinct regions positioned at matching surface areas of the skeletal muscle actin molecule from arthritic mice and patients with RA. Moreover, molecular dynamics simulations revealed that these areas, here coined hotspots, are important for the stability of the actin molecule and its capacity to generate filaments and interact with myosin. Together, these data demonstrate how oxidative modifications on actin promote muscle weakness in RA patients and may provide novel leads for targeted therapeutic treatment to improve muscle function.

Place, publisher, year, edition, pages
2019. Vol. 4, no 9, article id e126347
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Physiology
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URN: urn:nbn:se:su:diva-169261DOI: 10.1172/jci.insight.126347ISI: 000466814100015PubMedID: 30920392OAI: oai:DiVA.org:su-169261DiVA, id: diva2:1323471
Available from: 2019-06-12 Created: 2019-06-12 Last updated: 2019-06-12Bibliographically approved

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Aresh, BejanOlsson, KarlLundberg, Tommy R.Yamada, TakashiKarlsson, Björn C. G.Karlsson, Roger
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Department of Molecular Biosciences, The Wenner-Gren Institute
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