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Insect hemolymph coagulation: Kinetics of classically and non-classically secreted clotting factors
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.ORCID iD: 0000-0003-1117-9125
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
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Number of Authors: 82019 (English)In: Insect Biochemistry and Molecular Biology, ISSN 0965-1748, E-ISSN 1879-0240, Vol. 109, p. 63-71Article in journal (Refereed) Published
Abstract [en]

In most insects, hemolymph coagulation, which is analogous to mammalian blood clotting, involves close collaboration between humoral and cellular components. To gain insights into the secretion of cellular clotting factors, we created tagged versions of three different clotting factors. Our focus was on factors which are released in a non-classical manner and to characterize them in comparison to a protein that is classically released, namely Glutactin (Glt). Transglutaminase-A (Tg) and Prophenoloxidase 2 (PPO2), both of which lack signal peptide sequences, have been previously demonstrated to be released from plasmatocytes and crystal cells (CCs) respectively, the two hemocyte classes in naive larvae. We found that at the molecular level, Tg secretion resembles the release of tissue transglutaminase in mammals. Specifically, Drosophila Tg is associated with vesicular membranes and remains membrane-bound after release, in contrast to Glt, which we found localizes to a different class of vesicles and is integrated into clot fibers. PPO2 on the other hand, is set free from CCs through cytolysis. We confirm that PPO2 is a central component of the cytosolic crystals and find that the distribution of PPO2 appears to vary across crystals and cells. We propose a tentative scheme for the secretory events during early and late hemolymph coagulation.

Place, publisher, year, edition, pages
2019. Vol. 109, p. 63-71
Keywords [en]
Drosophila melanogaster, Wounding, Clotting, Secretion, Non-classical secretion, Transglutaminase, Glutactin, Prophenoloxidase, Bacteria, Defense, Septic injury, Hemolymph, Coagulation
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-170102DOI: 10.1016/j.ibmb.2019.04.007ISI: 000470192100007PubMedID: 30974174OAI: oai:DiVA.org:su-170102DiVA, id: diva2:1334427
Available from: 2019-07-02 Created: 2019-07-02 Last updated: 2019-07-02Bibliographically approved

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Schmid, Martin R.Dziedziech, AlexisArefin, BadrulAkhter, MuniraTheopold, Ulrich
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Department of Molecular Biosciences, The Wenner-Gren Institute
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