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Longitudinal studies of putative growth hormone (GH) biomarkers and hematological and steroidal parameters in relation to 2 weeks administration of human recombinant GH
Stockholm University, Faculty of Science, Department of Environmental Science and Analytical Chemistry.ORCID iD: 0000-0002-2485-4276
(English)Manuscript (preprint) (Other academic)
Abstract [en]

Detection of low doses of recombinant growth hormone is a challenge in anti-doping testing. Future testing leads toward longitudinal monitoring of IGF-I and P-III-NP in an endocrine module. Additional biomarkers, for example vitamin D binding protein, alpha-HS-glycoprotein, fibronectin 1 and decoin have been identified in different omics studies. Here we have longitudinally studied the usefulness of these putative biomarkers in relation to two weeks administration of low doses of recombinant growth hormone in healthy male volunteers. Moreover, we studied the hematological parameters included in the athlete biological passport as well as serum concentration of dihydrotestosterone-sulfate. Alpha-HS-glycoprotein decreased 25 % up to three weeks after treatment period (p˂0.05), whereas fibronectin increased 20 % during the treatment (p˂0.05). The addition of these two biomarkers increased the likelihood to detect recombinant growth hormone intake based on individual calculated thresholds in some of the participants as compared to the GH2000 score. A minor 10 % increase (p=0.003) in reticulocytes percentage and OFF-score was observed, but the changes did not lead to any atypical profile based on individual passport thresholds. Vitamin D binding protein, decoin and dihydrotestosterone-sulfate were not affected by recombinant growth hormone. Dihydrotestosterone-sulfate was negatively correlated with the IGF-I biomarker at baseline (R=-0.50, p=0.003) and post dose (R=-0.59, p=0.01). In conclusion we have verified Alpha-HS-glycoprotein and fibronectin 1 as promising future biomarkers for detecting low doses of recombinant growth hormone and further studies of these proteins are encouraged.

Keywords [en]
doping, growth hormone, IGF-I, fibronectin 1, alpha-HS-glycoprotein, DHT, athlete biological passport
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
URN: urn:nbn:se:su:diva-172565OAI: oai:DiVA.org:su-172565DiVA, id: diva2:1348246
Available from: 2019-09-03 Created: 2019-09-03 Last updated: 2019-09-04Bibliographically approved
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CiteExportLink to record
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