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Profiling surface proteins on individual exosomes using a proximity barcoding assay
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab). Uppsala University, Sweden.ORCID iD: 0000-0003-0293-9057
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Number of Authors: 152019 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 10, article id 3854Article in journal (Refereed) Published
Abstract [en]

Exosomes have been implicated in numerous biological processes, and they may serve as important disease markers. Surface proteins on exosomes carry information about their tissues of origin. Because of the heterogeneity of exosomes it is desirable to investigate them individually, but this has so far remained impractical. Here, we demonstrate a proximity-dependent barcoding assay to profile surface proteins of individual exosomes using antibody-DNA conjugates and next-generation sequencing. We first validate the method using artificial streptavidin-oligonucleotide complexes, followed by analysis of the variable composition of surface proteins on individual exosomes, derived from human body fluids or cell culture media. Exosomes from different sources are characterized by the presence of specific combinations of surface proteins and their abundance, allowing exosomes to be separately quantified in mixed samples to serve as markers for tissue-specific engagement in disease.

Place, publisher, year, edition, pages
2019. Vol. 10, article id 3854
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Biological Sciences
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URN: urn:nbn:se:su:diva-173111DOI: 10.1038/s41467-019-11486-1ISI: 000482567200002PubMedID: 31451692OAI: oai:DiVA.org:su-173111DiVA, id: diva2:1359223
Available from: 2019-10-08 Created: 2019-10-08 Last updated: 2019-10-09Bibliographically approved

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Wu, DiSun, YuQian, XiaoyanNilsson, Mats
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