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Insights on symbiotic diatom diazotroph associations in the South China Sea by targeted microarray analysis of three symbiont strains
Stockholm University, Faculty of Science, Department of Ecology, Environment and Plant Sciences.
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Abstract [en]

Three heterocystous cyanobacterial strains (two Richelia intracellularis: RintHH01, RintRC01, Calothrix rhizosoleniae: CalSC01) are known to form highly specific, intimate associations with several lineages of diatoms. Collectively these symbioses are unique since the cellular location varies from internal, partially integrated, and fully external; hence the immediate environment of the symbiont differs. We investigated the environmental gene expression levels of the three strains using a targeted mRNA microarray comprised of 748 genes on environmental samples collected in the South China Sea. Approximately half of the total genes (47%, 354 of the 748 genes) were expressed above background. The most highly transcribed genes were involved in photosynthesis, N2 fixation and potassium homeostasis, and strain differences were identifiable. The most important environmental parameters on the symbiont gene expression levels were fluorescence, temperature and beam transmission. However, salinity and oxygen impacted gene expression levels of one symbiont strain, RintHH01 differently (positively), possibly due to its different cellular location. Our results suggest that differences in gene expression patterns and environmental conditions influence the three closely related symbiont strains, and are likely related to their cellular location in their respective host diatoms.

Keywords [en]
cyanobacteria, DDA, Richelia, Calothrix, symbiosis, gene expression, nitrogen fixation, nifH, microarray, Mekong River, South China Sea
National Category
Ecology
Research subject
Marine Ecology
Identifiers
URN: urn:nbn:se:su:diva-174732OAI: oai:DiVA.org:su-174732DiVA, id: diva2:1373546
Available from: 2019-11-27 Created: 2019-11-27 Last updated: 2019-12-03Bibliographically approved
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Stenegren, MarcusFoster, Rachel A.
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