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Homogeneous Differential Magnetic Assay
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Number of Authors: 112019 (English)In: Acs Sensors, ISSN 2379-3694, Vol. 4, no 9, p. 2381-2388Article in journal (Refereed) Published
Abstract [en]

Assays are widely used for detection of various targets, including pathogens, drugs, and toxins. Homogeneous assays are promising for the realization of point-of-care diagnostics as they do not require separation, immobilization, or washing steps. For low concentrations of target molecules, the speed and sensitivity of homogeneous assays have hitherto been limited by slow binding kinetics, time-consuming amplification steps, and the presence of a high background signal. Here, we present a homogeneous differential magnetic assay that utilizes a differential magnetic readout that eliminates previous limitations of homogeneous assays. The assay uses a gradiometer sensor configuration combined with precise microfluidic sample handling. This enables simultaneous differential measurement sample containing a synthesized Vibrio cholerae target and a negative control sample, which reduces the background signal and increases the readout speed. Very low concentrations of targets down to femtomolar levels are thus detectable without any additional amplification of the number of targets. Our homogeneous differential magnetic assay method opens new possibilities for rapid and highly sensitive diagnostics at the point of care.

Place, publisher, year, edition, pages
2019. Vol. 4, no 9, p. 2381-2388
Keywords [en]
homogeneous differential magnetic assay, magnetic nanoparticle, volumetric detection, rolling circle amplification, Brownian relaxation, binding kinetics
National Category
Biological Sciences Chemical Sciences
Identifiers
URN: urn:nbn:se:su:diva-175879DOI: 10.1021/acssensors.9b00969ISI: 000488424100021PubMedID: 31397152OAI: oai:DiVA.org:su-175879DiVA, id: diva2:1374623
Available from: 2019-12-02 Created: 2019-12-02 Last updated: 2019-12-02Bibliographically approved

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Sepehri, SobhanJohansson, ChristerNilsson, MatsStrømme, Maria
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Department of Biochemistry and BiophysicsScience for Life Laboratory (SciLifeLab)
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