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PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera
Stockholm University, Faculty of Science, Department of Zoology.
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Number of Authors: 52016 (English)In: ZooKeys, ISSN 1313-2989, E-ISSN 1313-2970, no 596, p. 129-141Article in journal (Refereed) Published
Abstract [en]

We report primer pairs for 30 new gene regions in the nuclear genomes of Lepidoptera that can be amplified using a standard PCR protocol. The new primers were tested across diverse Lepidoptera, including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of Lepidoptera. We feel that a PCR-based approach still has its place in molecular systematic studies of Lepidoptera, particularly at the intrafamilial level, and our new set of primers now provides a route to generating phylogenomic datasets using traditional methods.

Place, publisher, year, edition, pages
2016. no 596, p. 129-141
Keywords [en]
Molecular systematics, Lepidoptera, phylogenomics, phylogenetics
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-176157DOI: 10.3897/zookeys.596.8399ISI: 000378055700009PubMedID: 27408580OAI: oai:DiVA.org:su-176157DiVA, id: diva2:1389448
Available from: 2020-01-30 Created: 2020-01-30 Last updated: 2020-01-30Bibliographically approved

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