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Proton-transfer pathways in the mitochondrial S. cerevisiae cytochrome c oxidase
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
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Number of Authors: 72019 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, article id 20207Article in journal (Refereed) Published
Abstract [en]

In cytochrome c oxidase (CytcO) reduction of O-2 to water is linked to uptake of eight protons from the negative side of the membrane: four are substrate protons used to form water and four are pumped across the membrane. In bacterial oxidases, the substrate protons are taken up through the K and the D proton pathways, while the pumped protons are transferred through the D pathway. On the basis of studies with CytcO isolated from bovine heart mitochondria, it was suggested that in mitochondrial CytcOs the pumped protons are transferred though a third proton pathway, the H pathway, rather than through the D pathway. Here, we studied these reactions in S. cerevisiae CytcO, which serves as a model of the mammalian counterpart. We analyzed the effect of mutations in the D (Asn99Asp and Ile67Asn) and H pathways (Ser382Ala and Ser458Ala) and investigated the kinetics of electron and proton transfer during the reaction of the reduced CytcO with O-2. No effects were observed with the H pathway variants while in the D pathway variants the functional effects were similar to those observed with the R. sphaeroides CytcO. The data indicate that the S. cerevisiae CytcO uses the D pathway for proton uptake and presumably also for proton pumping.

Place, publisher, year, edition, pages
2019. Vol. 9, article id 20207
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Biological Sciences
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URN: urn:nbn:se:su:diva-179700DOI: 10.1038/s41598-019-56648-9ISI: 000509347900012PubMedID: 31882860OAI: oai:DiVA.org:su-179700DiVA, id: diva2:1412090
Available from: 2020-03-05 Created: 2020-03-05 Last updated: 2020-03-05Bibliographically approved

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Björck, Markus L.Vilhjálmsdóttir, JóhannaHartley, Andrew M.Meunier, BrigitteNäsvik Öjemyr, LindaMarechal, AmandineBrzezinski, Peter
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