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Kinetic coupling of the respiratory chain with ATP synthase, but not proton gradients, drives ATP production in cristae membranes
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. (Prof Martin Ott)
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
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Number of Authors: 82020 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 117, no 5, p. 2412-2421Article in journal (Refereed) Published
Abstract [en]

Mitochondria have a characteristic ultrastructure with invaginations of the inner membrane called cristae that contain the protein complexes of the oxidative phosphorylation system. How this particular morphology of the respiratory membrane impacts energy conversion is currently unknown. One proposed role of cristae formation is to facilitate the establishment of local proton gradients to fuel ATP synthesis. Here, we determined the local pH values at defined sublocations within mitochondria of respiring yeast cells by fusing a pH-sensitive GFP to proteins residing in different mitochondrial subcompartments. Only a small proton gradient was detected over the inner membrane in wild type or cristae-lacking cells. Conversely, the obtained pH values did barely permit ATP synthesis in a reconstituted system containing purified yeast F1F0 ATP synthase, although, thermodynamically, a sufficiently high driving force was applied. At higher driving forces, where robust ATP synthesis was observed, a P-side pH value of 6 increased the ATP synthesis rate 3-fold compared to pH 7. In contrast, when ATP synthase was coreconstituted with an active proton-translocating cytochrome oxidase, ATP synthesis readily occurred at the measured, physiological pH values. Our study thus reveals that the morphology of the inner membrane does not influence the subcompartmental pH values and is not necessary for robust oxidative phosphorylation in mitochondria. Instead, it is likely that the dense packing of the oxidative phosphorylation complexes in the cristae membranes assists kinetic coupling between proton pumping and ATP synthesis.

Place, publisher, year, edition, pages
2020. Vol. 117, no 5, p. 2412-2421
Keywords [en]
mitochondria, ATP synthesis, cristae, energy conversion, kinetic coupling
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-179519DOI: 10.1073/pnas.1917968117ISI: 000512340900032PubMedID: 31964824OAI: oai:DiVA.org:su-179519DiVA, id: diva2:1412940
Available from: 2020-03-09 Created: 2020-03-09 Last updated: 2020-03-30

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Toth, AlexandraStoldt, StefanJakobs, Stefanvon Ballmoos, ChristophOtt, Martin
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