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On the origin of the widespread self-compatible allotetraploid Capsella bursa-pastoris (Brassicaceae)
Stockholm University, Faculty of Science, Department of Ecology, Environment and Plant Sciences. Stockholm University, Science for Life Laboratory (SciLifeLab).ORCID iD: 0000-0002-7212-813X
Stockholm University, Faculty of Science, Department of Ecology, Environment and Plant Sciences. Stockholm University, Science for Life Laboratory (SciLifeLab).ORCID iD: 0000-0002-7378-4673
Stockholm University, Faculty of Science, Department of Ecology, Environment and Plant Sciences. Stockholm University, Science for Life Laboratory (SciLifeLab).
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Abstract [en]

Polyploidy, or whole genome duplication, is common in plants and can contribute to rapid evolution of reproductive barriers and thus speciation. An important barrier to polyploid establishment and persistence is a lack of compatible mates. Because self-compatibility alleviates this problem, it has long been hypothesized that there should be an association between polyploidy and self-compatibility (SC), but empirical support for this prediction has been mixed. Here, we investigate whether the molecular makeup of the Brassicaceae self-incompatibility (SI) system, and specifically dominance relationships among S-haplotypes mediated by small RNAs, could facilitate the loss of SI in allopolyploid crucifers. We focus on the allotetraploid species Capsella bursa-pastoris, which formed ~300 kya by hybridization and whole genome duplication involving progenitors from the lineages of Capsella orientalis and Capsella grandiflora. We conduct targeted long-read sequencing to assemble and analyze eight full-length S-locus haplotypes, representing both homeologous subgenomes of C. bursa-pastoris. We further analyze small RNA (sRNA) sequencing data from flower buds to identify candidate dominance modifiers. We find that the C. orientalis-derived S-haplotypes of C. bursa-pastoris harbor truncated versions of the male SI specificity gene SCR and express a conserved sRNA-based candidatedominance modifier with a target in the C. grandiflora-derivedS-haplotype. These results suggest that pollen-level dominance may have facilitated loss of SI in C. bursa-pastoris. Finally, we report on the spontaneous somatic tetraploidization and production of tetraploid offspring after a wide cross between C. orientalis and C. grandiflora, and discuss the implications of this finding on the mode of formation of this widespread weed. 

Keywords [en]
allopolyploid, artificial hybrid, dominance modifier, long-read sequencing, mating system, polyploidy, self-fertilization, S-locus
National Category
Evolutionary Biology Genetics
Research subject
Ecology and Evolution
Identifiers
URN: urn:nbn:se:su:diva-179375OAI: oai:DiVA.org:su-179375DiVA, id: diva2:1415304
Funder
Swedish Research Council, 621-2013-4320Science for Life Laboratory - a national resource center for high-throughput molecular bioscienceAvailable from: 2020-03-18 Created: 2020-03-18 Last updated: 2020-03-19Bibliographically approved
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Bachmann, Jörg A.Tedder, AndrewSteige, Kim A.Slotte, Tanja
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