We perform the calibration of the X-ray luminosity-mass scaling relation on a sample of 344 CODEX clusters with z < 0.66 using the dynamics of their member galaxies. Spectroscopic follow-up measurements have been obtained from the SPIDERS survey, leading to a sample of 6658 red member galaxies. We use the Jeans equation to calculate halo masses, assuming an NFW mass profile and analysing a broad range of anisotropy profiles. With a scaling relation of the form L-X proportional to A(X)M(200c)(BX) E(z)(2)(1 + z)(gamma x), we find best-fitting parameters A(X) = 0.62(-0.06)(+0.05) (+/- 0.06) x 10(44) erg s(-)(1), B-X = 2.35(-0.18)(+0.21)(+/- 0.09), gamma(X) = -2.77(-1.05)(+1.06)(+/- 0.79), where we include systematic uncertainties in parentheses and for a pivot mass and redshift of 3 x 10(14) M-circle dot and 0.16, respectively. We compare our constraints with previous results, and we combine our sample with the SPT SZE-selected cluster subsample observed with XMM-Newton extending the validity of our results to a wider range of redshifts and cluster masses.