Respect for human rights is one of the main conditions for European Union (EU) cooperation with third countries. When human rights are gravely violated, the EU can apply a variety of restrictive measures ranging from issuing sanctions threats to imposing economic sanctions. However, can such measures impact the subsequent human rights situation in the target country, and under what conditions? Using time-series cross-sectional data accounting for eleven post-Soviet states over a period of twenty-six years (1991–2016), this article demonstrates that the impact of sanctions on the target’s human rights situation depends on two factors – the type of sanction applied and the extent of the target’s linkage with alternative regional powers. In the absence of regional alternative support, sanctions in the form of arms embargoes and aid suspension demonstrate a positive effect on the human rights situation.