The role of the N-terminal domain of chloroplast targeting peptides in organellar protein import and miss-sorting
2006 (English)In: FEBS Letters, ISSN 0014-5793, Vol. 580, no 16, 3966-3972 p.Article in journal (Refereed) Published
We have analysed 385 mitochondrial and 567 chloroplastic signal sequences of proteins found in the organellar proteomes of Arabidopsis thaliana. Despite overall similarities, the first 16 residues of transit peptides differ remarkably. To test the hypothesis that the N-terminally truncated transit peptides would redirect chloroplastic precursor proteins to mitochondria, we studied import of the N-terminal deletion mutants of ELIP, PetC and Lhcb2.1. The results show that the deletion mutants were neither imported into chloroplasts nor miss-targeted to mitochondria in vitro and in vivo, showing that the entire transit peptide is necessary for correct targeting as well as miss-sorting.
Place, publisher, year, edition, pages
2006. Vol. 580, no 16, 3966-3972 p.
Chloroplast; Mitochondria; Targeting peptide; Protein import; Miss-sorting
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
IdentifiersURN: urn:nbn:se:su:diva-8750DOI: 10.1016/j.febslet.2006.06.018PubMedID: 16806197OAI: oai:DiVA.org:su-8750DiVA: diva2:175269